Bolton manager Owen Coyle is set to give goalkeeper Adam Bogdan his debut, resting Jussi Jaaskelainen.
Matt Taylor, a former Portsmouth favourite, may be given a first start of the season against his old rivals.
MATCH FACTS
Head-to-head
• Bolton are unbeaten in their last seven meetings with Southampton.
• The Trotters lead the overall head-to-head by 16 wins to 11.
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Southampton
• Southampton were knocked out by Premier League opposition at this stage last season, losing 2-1 at home to Birmingham City.
• Southampton have yet to win in the league, picking up one point from two games.
• Radhi Jaidi could be playing against one of his former clubs.
Bolton
• Bolton, runners-up in 1995 and 2004, reached the fourth round last season before losing to Chelsea.
• They have made an unbeaten start to the Premier League season, picking up four points from two games.
• If he plays, Matt Taylor will make his 100th appearance for Bolton.
Source: BBC Sport
Source: BBC Sport